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Two-dimensional gel electrophoretic analysis of rat sperm membrane interaction with cauda epididymal fluid
Two-dimensional gel electrophoretic analysis rat sperm membrane interaction cauda epididymal fluid
2009/2/23
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used to analyze the polypeptide composition of rat cauda epididymal fluid, blood serum and membrane-enriched fractions of caput, corp...
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
Modification of the rat sperm flagellar plasma membrane during maturation in the epididymis
Modification rat sperm flagellar plasma membrane maturation epididymis
2009/2/23
Previously, we demonstrated that surface radiolabeling of rat epididymal spermatozoa by lactoperoxidase-catalyzed iodination reveals a major component with an apparent molecular weight of 26,000 to ...
Turnover of Monocytoid Cells Within the Limiting Membrane of Rat Seminiferous Tubules
testis seminiferous tubules limiting membrane mononuclear leukocyte cell renewal
2009/2/19
The mode of renewal of mononuclear leukocytes found between the myoid cells forming the limiting membrane of rat seminiferous tubules was investigated by histometric methods. The average number of...
Acrosome reaction in dog sperm is induced by a membrane-localized progesterone receptor
Canine spermatozoa prostatic fluid
2009/2/18
The aim of this study was to investigate whether the dog sperm acrosome reaction can be induced by progesterone and whether the action of progesterone is mediated by binding of progesterone to a r...
Exposure of human, boar, or bull sperm to a synthetic peptide increases binding to an egg-membrane substrate
human boar bull sperm synthetic peptide increases egg-membrane substrate
2009/2/18
We evaluated the effects of in vitro exposure of sperm to synthetic FertPlus peptide, which represents a 60-amino acid sequence within rat prosaposin, using a microwell sperm-binding assay (SBA), ...
Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation
Extender components surfactants boar sperm function membrane behavior cryopreservation
2009/2/18
To determine how the individual components of extenders affected boar sperm function and membrane structure and to test a new surfactant's cryoprotective ability, boar sperm were cryopreserved in ...
Altered basement membrane synthesis in the testis after tissue injury
Efferent duct ligation gene expression protein synthesis
2009/2/17
The basement membrane plays an important role in maintaining the structural and functional integrity of tissues. Altered basement membrane structure has been associated with severe functional impair...
Age-related increases in basement membrane thickness have been noted in many tissues, including the testis. The current investigation examined the morphology of the basement membrane in the aged B...
Two-dimensional electrophoretic profile of human sperm membrane proteins
Human spermatozoa membrane proteins two-dimensional electrophoresis contraceptive vaccine
2009/2/13
The purpose of this study was to characterize highly enriched human spermatozoa membrane proteins by two-dimensional electrophoresis and computer image analysis. Sperm membrane proteins were extract...
Nanovid microscopy for assessing sperm membrane changes induced by in vitro capacitating and acrosomal reacting procedures
Spermatozoa colloidal gold videomicroscopy
2009/2/12
This study was to verify the usefulness of Nanovid microscopy techniques for evaluating induced modifications in bovine spermatozoal membranes. Frozen thawed bovine sperm were labeled with 20-nm c...
Atrial natriuretic factor inhibits the phosphorylation of protein kinase C in plasma membrane preparations of cultured Leydig tumor cells
Atrial natriuretic factor protein kinase C Leydig tumor cells cGMP plasma membranes
2009/2/12
The peptide hormone atrial natriuretic factor (ANF) exerts its effect in a receptor-mediated fashion and the membrane-bound form of guanylate cyclase represents a biologically active ANF receptor;...
Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid
Sperm PAF PTX cryoprotection lipid peroxidation
2009/2/12
Sperm membrane damage during cryopreservation reduces the recovery of motile sperm. The present study investigates changes in sperm motility and membrane lipid peroxidation (LPO) in response to two ...
Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant
Human sperm cryopreservation cryoprotectant sublethal cryodamage lipid peroxidation membrane stress
2009/2/12
One effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. We hypothesized two modes of suble...
Sertoli cell plasma membrane polypeptides involved in spermatogenic cell-Sertoli cell adhesion
Sertoli cell spermatogenic cell-Sertoli cell adhesion
2009/2/10
This study concerns Sertoli cell-spermatogenic cell adhesive interactions in the seminiferous tubule. Sertoli cell surface polypeptides involved in germ cell-Sertoli cell adhesion were identified ...