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Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro
equine spermatozoa oviductal epithelial cells zonae pellucidae
2009/2/13
Two bioassays were used to evaluate the interaction of fresh and cryopreserved equine semen with oviductal epithelial cells (OEC) and with the zona pellucida (ZP). Split ejaculates were either store...
Effects of lipoprotein, equine luteinizing hormone, equine follicle-stimulating hormone, and equine prolactin on equine testicular steroidogenesis in vitro
Stallion testes steroid estrogen
2009/2/13
A stallion testicular cell incubation system was developed and used to investigate the regulation of steroidogenesis in stallion testes. Cells isolated from testes of 2- to 4-year-old stallions (n = 6...
Validation of an acrosomal stain for equine sperm that differentiates between living and dead sperm
acrosomal stain equine sperm living and dead sperm
2009/2/12
An acrosomal staining technique that can differentiate between living and dead sperm was developed for equine sperm. The fluoresceinated lectin Pisum sativum agglutinin (FITC-PSA) was used to iden...
The testis as a major source of circulating inhibins in the male equine fetus during the second half of gestation
Horse fetal tests inhibin A inhibin pro-C Sertoli
2008/12/30
Immunolocalization of the inhibin (a) and inhibin/activin (beta3A and betaB) subunit proteins in equine fetal testes was investigated to determine the ability of the fetal testis to produce inhibi...
Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential
Sperm osmotic stress horse cryopreservation glycerol
2008/12/29
Osmotic stress attributed to differences in the relative permeability of cryoprotectants, such as glycerol and water, appears to be an important factor in cryodamage. The objective of this study was...
The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation
reactive oxygen species equine sperm motility viability
2008/12/25
The objective of this study was to examine the influence of reactive oxygen species (ROS), generated through the use of the xanthine (X)-xanthine oxidase (XO) system, on equine sperm motility, via...
Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa
DNA damage oxidative stress antioxidants horse sperm
2008/12/19
The objective of this study was to examine the effect of reactive oxygen species (ROS) and cryopreservation on DNA fragmentation of equine spermatozoa. In experiment 1, equine spermatozoa were incub...
Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C1l-BODIPY581/591
lipid peroxidation equine spermatozoa lipophilic fluorescent dye C1l-BODIPY581/591
2008/12/2
The lipophilic fluorescent probe, 4,4-difluoro-5-(4-phenyl-1 ,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY581/591) was used to evaluate changes in lipid peroxidation in ...
The testis as a major source of circulating inhibins in the male equine fetus during the second half of gestation
testis major source circulating inhibins male equine fetus gestation
2008/12/2
Immunolocalization of the inhibin (a) and inhibin/activin (beta3A and betaB) subunit proteins in equine fetal testes was investigated to determine the ability of the fetal testis to produce inhibins...
Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis
DNA damage equine spermatozoa single-cell gel electrophoresis
2008/12/1
Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at ...