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Age-related changes in the concentration of cytosolic androgen receptors in the epididymis, vas deferens and seminal vesicle of maturing male mice
Age-related changes cytosolic androgen receptors epididymis
2009/2/23
In this study, changes in the number of androgen binding sites that occur in cytosols of epididymis, vas deferens and seminal vesicle of mice from 10 to 90 days of age are described. Specific satura...
Evidence for age-dependent changes in Sertoli cell androgen receptor concentration
age-dependent changes Sertoli cell receptor concentration
2009/2/23
Cytosol and nuclear receptor concentrations in Sertoli cells isolated from the testes of 15-, 25-, and 35-day-old rats were measured using hydroxylapatite separation procedures. In these cells the m...
Repair of experimental varicoceles in the rat. Long-term effects on testicular blood flow and temperature and cauda epididymidal sperm concentration and motility
varicocele varicocele repair rat testis
2009/2/23
The effects of varicocele and varicocele repair on testicular blood flow, temperature, sperm counts, and sperm motility were assessed in adult male rats. The duration of the experimental varicocele ...
Determination of the steady-state intracellular chloride concentration in capacitated human spermatozoa
Methoxy-N-ethylquinolonium N-(6-methoxyquinolyl)
2009/2/18
Chloride channels participate in the mammalian sperm acrosome reaction (AR). However, the mechanism by which sperm regulate intracellular Cl- concentration ([Cl-]i) is not known. The steady-state ...
Evaluation of computer-assisted semen analysis（CASA）with IDENT stain to determine sperm concentration
Sperm count sperm density CASA
2009/2/16
This study was undertaken to compare a new fluorescent stain-based computer-assisted semen analysis (CASA) system (IDENT) for determining human sperm concentration to the manual hemacytometric met...
Methodological issues in the analysis of human sperm concentration data
Sperm concentration log transformation multiple sampling variation statistical power
2009/2/16
We examined two methodological issues in the analysis of sperm concentration data using a large database of sperm concentrations in healthy men that were collected at the University of Washington....
Selenium supplementation enhances the element concentration in blood and seminal fluid but does not change the spermatozoal quality characteristics in subfertile men
Selenium supplementation element concentration
2009/2/13
The objective of this study was to evaluate the effect of selenium (Se) supplementation on Se concentration and glutathione peroxidase (GSH-Px) activity in blood components and seminal fluid and o...
Intratesticular testosterone concentration following intratunical administration in the hypogonadal animal
Testosterone tunica vaginalis hypogonadism infertility
2009/2/13
The serum and intratesticular testosterone concentration (ITC) after testosterone administration in the tunica vaginalis (TV) sac compared to parenteral administration was studied in 20 dogs that were...
Segment-Specific Decrease of Both Catecholamine Concentration and Acetylcholinesterase Activity Are Accompanied by Nerve Refinement in the Rat Cauda Epididymis During Sexual Maturation
Segment-Specific Catecholamine Concentration Acetylcholinesterase Activity Nerve Refinement Rat
2008/12/30
In the present work, histochemical and biochemical studies were conducted to analyze changes in the pattern of autonomic innervation during sexual maturation, using the rat epididymis as a model....
Evaluation of flow cytometric methods to measure human sperm concentration
Semen cell count DNA staining fluorospheres hemocytometry
2008/12/29
Human sperm concentration is usually assessed using hemocytometry (HM). However, external and internal quality control schemes have shown that the accuracy of this method is low overall. Flow cyto...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Seminal Plasma Cobalamin Significantly Correlates With Sperm Concentration in Men Undergoing IVF or ICSI Procedures
Assisted reproduction folate homocysteine semen analysis
2008/12/24
Mild hyperhomocysteinemia is caused by B vitamin deficiencies. We hypothesize that these biochemical derangements detrimentally affect spermatogenesis. Therefore, the aim of this study was to inv...
Serotonin Concentration, Synthesis, Cell Origin, and Targets in the Rat Caput Epididymis During Sexual Maturation and Variations Associated With Adult Mating Status：Morphological and Biochemical Studies
5-hydroxytryptamine tryptophan hydroxylase neuroendocrine cells male reproduction serotonin receptor spermatozoa
2008/12/24
The caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical...
Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer（Cervus elaphus hispanicus）Epididymal Spermatozoa: Effects of Concentration and Temperature of Addition
Ethylene glycol glycerol propylene glycol sperm cryopreservation
2008/12/24
With the aim of finding an ideal cryoprotectant (CPA) in a suitable concentration for red deer epididymal spermatozoa cryopreservation, we evaluated the effects of the 3 most commonly used CPAs, ...
The Precision and Accuracy of Six Different Methods to Determine Sperm Concentration
Cytometry hemocytometry microcells spectrophotometry plate reader
2008/12/23
The development of new technologies and software that are routinely used in laboratories has now allowed for a more diverse novel range of methods to determine sperm concentrations more rapidly. The...