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构建了棉花抗黄萎病性状的关联群体和遗传分离群体,联合利用全基因组关联分析(GWAS)、QTL- Seq和转录组测序开展了棉花抗黄萎病基因发掘与KASP标记开发研究,获得了5个主效抗黄萎病QTL的候选基因区间,开发出10个可以用于陆地棉抗病性标记辅助选择育种的KASP标记,同时鉴定出8个抗黄萎病候选基因,这些基因主要参与植物基础免疫反应及广谱抗病性,推测基础免疫反应相关基因在抗、感品种间的gDNA&...
Scaphoideus titanus Ball, the main vector of Flavescence dorée phytoplasma, was monitored in Slovakia during 2013 and 2014. The species was present in the vineyards of eastern Slovakia but it was rare...
one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The ...
Myrobalan latent ringspot virus (MLRSV) was purified from the extract of infected Chenopodium quinoa Willd., using n-octanol, differential centrifugation, and saccharose gradient centrifugation. Ele...
Daughter tubers of volunteer potatoes were tested for their ability to maintain Clavibacter michiganensis subsp. sepedonicus (Cms). In different areas of the CR, volunteer potatoes were searched for i...
Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymer...
ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which ...
Hop downy mildew (Pseudoperonospora humuli) and hop powdery mildew (Podosphaera macularis) are the most important pathogens of hop (Humulus lupulus). The early detection and identification of these pa...
The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all ti...
The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultiva...
Four polyclonal and two monoclonal antibodies were prepared and tested to detect a quarantine pathogen of strawberry – Colletotrichum acutatum. Only one of them, polyclonal IgG K91, was sensitive enou...
Monoclonal antibody Mn-Cs1 with a high level of sensitivity and specificity for detection and determination of Clavibacter michiganensis subsp. sepedonicus was prepared. Strain C. m. subsp. sepedonicu...
In 2009 in Slovenia, an uncultivable cell-wall-less pathogenic bacterium of the class Mollicutes - a phytoplasma belonging to the 16SrV (Elm yellows) group was found in the mosaic leafhopper (Orientus...
In the process of safety assessments of genetically modified (GM) crops, particular attention should be paid to their unintended effects. This study was focused on detection of unintended effects in g...
A multiplex RT-PCR procedure was developed for the simultaneous detection of Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus (PRSV) from Cucurbitaceae cr...

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