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Molecular typing of Mycobacterium bovis isolated in the first outbreak of bovine tuberculosis in the Azores Islands: a case report
spoligotyping MIRU-VNTR typing Mycobacterium bovis
2015/4/8
Twenty Mycobacterium bovis isolates from a first reported outbreak in the Azores Islands were differentiated into four spoligotypes: SB0119 (45%), SB0121 (40%), SB1264 (10%) and SB1090 (5%) and into t...
Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001–2003
Clostridium perfringens multiplex PCR β2-toxin pig enteritis
2015/3/5
Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isol...
Gene typing of the colonisation factors F18 of Escherichia coli isolated from piglets suffering from post-weaning oedema disease
oedema disease pig colonisation factors Stx2e toxin PCR
2015/1/26
Production of verotoxin Stx2e and expression of F18 and K88 colonisation factors were investigated in 222 strains of Escherichia coli isolated from weaned piglets. Sixty-two, 30, and 11 of the 129 ver...
Implications for the use of horse hair roots as a DNA source for microsatellite typing
hair follicles short tandem repeats multiplex PCR
2015/6/1
Hair roots are a very attractive source of DNA for microsatellite-based parentage control of breeding animals. However, unlike blood samples, irregular DNA typing results have been observed in assays ...
Microsatellite DNA Typing Using 16 Markers for Parentage Verification of the Korean Native Horse
Allele Korean Native Horse Microsatellite DNA Parentage Verification
2016/4/15
This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite marker...
SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model
SSCP SNaPSho Mlo SNP DNA marker barley primer extension
2014/3/19
We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP
detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP p...