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南京农业大学农学院《Nature Communica》在线发表万建民课题组“A toxin-antidote system contributes to interspecific reproductive isolation in rice”（图）
万建民 基因 栽培
2024/6/11
2023年11月19日，南京农业大学万建民院士团队在Nature Communications在线发表了题为“A toxin-antidote system contributes to interspecific reproductive isolation in rice”的研究论文，揭示了两个紧密连锁的基因组成一个毒药-解毒（toxin-antidote）元件，可选择性败育不包含该元件的花粉...
Isolation of diploid and tetraploid cytotypes from mixoploids based on adventitious bud regeneration in Populus
Adventitious bud Colchicine Mixoploid Populus Tetraploid
2024/2/27
Somatic chromosome doubling usually results in the production of mixoploids, which are difficult to use in breeding programs. In this study, we developed a method to isolate diploid and tetraploid cyt...
Effects of 20-day litter weight on weaned piglets’ fighting behavior after group mixing and on heart rate variability in an isolation test
Fighting Behavior Isolation Test Skin Lesion Score Heart Rate Variability Autonomic Nervous System
2017/3/9
The objective of this study was to investigate the effect of 20-day litter weight on behavior and heart rate variability (HRV) of piglets under stress.
Effects of previous experience on total blood and free iodothyronine responses to isolation, restraint and shearing in sheep (Ovis aries)
sheep isolation restraint
2016/2/23
The aim of this study was to investigate a possible effect on thyroid hormone concentrations in peripheral blood in sheep submitted to isolation, restraint and shearing. Circulating T3, T4, fT3 and fT...
Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Buffalo Spermatogonial Stem Cell Germ Cell Marker Proliferation
2016/11/10
Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultu...
P歟ni鑛a M.: Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca)
germ cells isolation transplantation
2015/7/2
A practical technique for isolation and cryopreservation of tench (Tinca tinca) (Cyprinidae,
Teleostei) early stages of germ cells (GC), including spermatogonia and spermatocytes, is reported for the...
Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs
Theileria annulata theileriosis antitheilerials lactate dehydrogenase gene cloning
2015/4/15
Theileriosis is a serious animal disease that is transmitted by ticks. Theileria species are tick-borne obligate intracellular protozoan parasites that cause severe and mild infections in their hosts....
Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples
Listeria seasonal variation PCR-RFLP RAPD
2015/4/15
A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from Februar...
Isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens): a case report
dermatomycoses rhodotorulosis sertaconazol
2015/4/8
This paper reports the isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens). The microorganism was isolated from cutaneous lesions, identified by the comm...
First isolation of Mycobacterium genavense in a blue headed parrot (Pionus menstruus) imported from Surinam (South America) to the Czech Republic: a case report
zoonosis avian tuberculosis Psittaciformes
2015/4/7
In 2005 a Mycobacterium genavense infection was diagnosed in one blue-headed parrot (Pionus menstruus) imported from Surinam (South America), the first such incidence in the Czech Republic. The bird d...
Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003
IS901 IS1245 dnaJ zoonosis avian mycobacteriosis food safety ecology
2015/3/13
Between 1997 and 2003, in one herd of breeding pigs with 90 sows and two boars, positive and dubious responses to avian tuberculin were detected in 4 and 72 pigs, respectively. Pigs were examined usin...
Methods of mycobacterial DNA isolation from different biological material: a review
Johne’s disease Crohn’s disease zoonoses
2015/3/5
Mycobacteria cause serious infections in animals and human beings. Huge economic losses on farms are caused by selected species of this wide family. A high risk of transmission of infection from anima...
Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals
Bos taurus Sus scrofa Equus caballus cattle pig horse centromeres tandem repeats chromosomes FISH
2015/3/5
In this study, new classes of tandemly repeated DNA sequences in the centromeric regions of three farm animal species are reported. Pericentromeric regions of bovine chromosome X, porcine chromosome 1...
Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses
cell line MA-104 electron microscopy immunoperoxidase test ELISA monoclonal antibody electropherotype group A and C rotavirus RT-PCR
2015/3/5
Rotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Ro...
Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV
PR-proteinsNPAGENTobacco mosaic viru
2015/2/2
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (T...