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The selection of superior lines is extremely important to improve the utilization rate and economic value of bamboo. In this research, 120 anther-regenerated bamboo lines were planted in the field, an...
GhGPAT12和GhGPAT25为一对位于陆地棉A12/D12染色体上编码甘油-3-磷酸酰基转移酶(GPAT)的旁系同源基因。GhGPAT12/25在发育早期的花药中特异表达,其转录本主要富集于绒毡层和花粉外壁。真核表达实验表明GhGPAT12/25具有sn-2酰基转移酶活性。CRISPR/Cas9介导的基因敲除实验发现GhGPAT12和GhGPAT25存在功能冗余,两基因的同时敲除导致彻底的雄...
Pea (Pisum sativum L.) is a species for which there is no efficient method for the recovery of haploid plants yet. This research investigated the influence of various genotypes, nutrient media, and st...
The cytological mechanism of male sterility of Shaan-GMS, a natural mutant dominant genic male sterile (DGMS) line in Brassica napus L., is not well studied. Cytological observation was made on differ...
This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these struct...
Histological characteristics of globular shaped pollen callus (globules) developed from multicellular pollen grains were examined in rice anther culture (Oryza sativa L.). The ultrastructure of polle...
The formation of pollen callus was induced when rice anthers, which contained microspores in the mid-uninucleate and late-uninucleate stage, were cultured. The ultrastructure of the microspores was al...
Development of pollen cells as well as the fate of tapetal cells were studied by the glycol methacrylate semithin method for light microscopy. Sections including artificially produced images of pollen...
This study was carried out to verify the production of haploid plantlets through somatic embryogenesis of Bupleurum falcatum in anther culture (2n=16). Flowers with anthers at the uninucleate stage, l...
Induction of Haploid Plantlets by Anther Culture of Bupleurum falcatum L.
Doubled haploid (DH) lines by using an anther culture technique and conventional breeding (CB) lines of rice were compared for grain yield and palatability. No difference was found between the mean he...
Anther length, anther width, and number of engorged pollens were reported to have a relationship to cooling injury of rice. The effects of nitrogen application on anther size and number of pollens wer...
We determined the callus induction medium and the regeneration medium to efficiently utilize the anther culture technique in rice breeding. The medium containing N6 basal medium supplemented with 4 mg...
To improve the regeneration efficiency from anther culture of pearl millet (Pennisetum typhoideum Rich.), we studied the influence of the composition of culture media, stage of anther and low tempera...

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