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从仔猪粪样中分离鉴定奇异变形杆菌(Proteus mirabilis)的烈性噬菌体,并对其进行生物学特性分析和基因组分析。以Proteus mirabilis为宿主菌,用双层平板法从仔猪粪样中分离噬菌体。利用电子显微镜观察噬菌体大小和形态。提取噬菌体DNA,并使用Illumina Hiseq进行高通量测序,对基因组进行注释和分析。结果显示:分离到的奇异变形杆菌噬菌体命名为vB_PmiP_P59(P...
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中国科学院新疆生态与地理研究所“千人计划”入选者马剑英团队通过野外试验,选取古尔班通古特沙漠南缘三个不同灌木群落的6种共存C3和C4灌木,根据土壤水、地下水和植物茎木质部水稳定氢氧同位素特征(图1),分析了6种共存灌木水分来源的季节动态和种间差异。研究结果显示,不同群落所有C3和C4灌木具有相似的水分利用季节变化规律,即春季土壤表层水分充足时,所有灌木对表层土壤水利用率相对增高(> 40 %),而...
Differential responses of plant functional trait to grazing between two contrasting dominant C3 and C4 species in a typical steppe of Inner Mongolia, China
plant functional trait grazing between two dominant C3 and C4 species typical steppe of Inner Mongolia China
2015/2/4
Plant functional traits have been widely used to study the linkage between environmental drivers, trade-offs among different functions within a plant, and ecosystem structure and functioning. Here, th...
Changes in the abundance of C3/C4 species of Inner Mongolia grassland: evidence from isotopic composition of soil and vegetation
d13C carbon isotope discrimination crossover temperature geostatistics precipitation semivariogram soil organic carbon Suess effect wool
2015/2/4
Global warming, increasing CO2 concentration, and environmental disturbances affect grassland communities throughout the world. Here, we report on variations in the C3/C4 pattern of Inner Mongolian gr...
Large regional-scale variation in C3/C4 distribution pattern of Inner Mongolia steppe is revealed by grazer wool carbon isotope composition
Large regional-scale variation C3/C4 distribution pattern Inner Mongolia steppe grazer wool carbon isotope composition
2015/2/4
This work explored the spatial variation of C3/C4 distribution in the Inner Mongolia, P. R. China, steppe by geostatistical analysis of carbon isotope data of vegetation and sheep wool. Standing commu...
Carbon isotope discrimination of C3 vegetation in Central Asian grassland as related to long-term and short-term precipitation patterns
Carbon isotope discrimination C3 vegetation Central Asian grassland short-term precipitation patterns
2015/2/4
The relationship between carbon isotope discrimination (13 1) of C3 vegetation and long-term (30 years) and short-term (growing period) precipitation was investigated. Different species of Stipa, a do...
Effects of Temperature on CO2 Dependence of Gas Exchanges in C3 and C4 Crop Plants
C4 crop plant CO2 concentration CO2 exchange rate Stomatal conductance Temperature
2009/8/5
The effects of elevated CO2 in the atmosphere and the accompanied temperature rise predicted for the future on gas exchanges of two summer C3 (rice, soybean) and two C4 Japanese millet, finger millet)...
Relation between Leaf Gas Exchange Rate and Stomata:I. Stomatal frequency and guard cell length in C3 and C4 grass species
C3 and C4 Photosynthesis Gramineae Guard cell length Rice plants
2009/7/28
We measured stomatal frequency and guard cell length in the Gramineae and compared these components among the photosynthetic types and/or the subfamilies. The mean stomatal frequency of the Eragrostoi...
Expression of Photosynthesis-Related Genes during the Leaf Development of a C3 Plant Rice as Visualized by In Situ Hybridization
Bundle sheath Chlorophyll a/b-binding protein in situ hybridization Lamina joint Leaf development Mesophyll Rice Rubisco small subunit
2009/7/24
The expression of photosynthesis-related genes, rbcS (small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase) and cab (light-harvesting chlorophyll a/b-binding protein), in emerging rice lea...
Production of Intergeneric Hybrid Calli from C3 and C4 Species of Amaranthaceae through Protoplast Fusion
Amaranthus tricolor L Celosia cristata L C4 species C3 species Electrofusion
2009/7/23
Intergeneric somatic hybrid calli were obtained through electrofusion between protoplasts of Celosia cristata L. (C3 species) cell suspension and Amaranthus tricolor L. (C4 species) cotyledon. The hig...
Inheritance of C3-C4 Intermediate Photosynthesis in Reciprocal Hybrids between Moricandia arvensis (C3-C4) and Brassica oleracea (C3) that Differ in their Genome Constitution
Brassicaceae C3 species C3-C4 intermediate species CO2 exchange Glycine decarboxylase Leaf anatomy Photorespiration Reciprocal hybrids
2009/7/23
To elucidate the genetic mechanisms underlying C3-C4 intermediate photosynthesis, we investigated the structural and photosynthetic characteristics of leaves of reciprocal hybrids between the C3-C4 in...
Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice,a C3 Plant
Bundle sheath C3 photosynthesis Chlorophyll fluorescence Immuno-localization In situ hybridization Leaf development Leaf senescence Mesophyll
2009/7/23
Gene expression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) and small subunit (rbcS) in bundle sheath and mesophyll cells of rice, a C3 plant, was examined during...
Production of Raphanus sativus (C3)-Moricandia arvensis (C3-C4 intermediate) Monosomic and Disomic Addition Lines with Each Parental Cytoplasmic Background and their Photorespiratory Characteristics
C3-C4 intermediate plant CO2 compensation point Disomic addition line (DAL) Monosomic addition line (MAL) Moricandia arvensis Photorespiration
2009/7/20
We are maintaining five Moricandia arvensis monosomic addition lines of Raphanus sativus carrying R. sativus cytoplasm (autoplasmic MALs) and twelve M. arvensis MALs of R. sativus carrying M. arvensis...
感染异育银鲫补体组分C3·C4 表达研究
异育银鲫 嗜水气单胞菌 补体组分C3、C4 表达
2009/6/9
[目的]了解补体组分在感染性鱼类疾病中的作用,为鱼类疾病的诊断和治疗提供参考。[方法]采用不同浓度(2×106、4×106
CFU/ml)的嗜水气单胞菌感染异育银鲫,利用半定量RT-PCR 技术检测感染不同时间后肝脏中补体组分C3 和C4的表达量。[结果]感
染后4、5 h时,异育银鲫肝脏中C3的表达量均显著高于对照组。在感染后4 h时,不同浓度组之间C3 的表达量无显著差异。而在感
染...