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Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Quantitative Analysis of Supported Membrane Composition Using the NanoSIMS
lipid bilayer SIMS NanoSIMS mixture gradient compositional analysis
2016/5/24
We have improved methods reported earlier [1] for sample preparation, imaging and quantifying components in supported lipid bilayers using high-resolution secondary ion mass spectrometry performed wit...
Quantitative Analysis by the Point-Centered Quarter Method
Quantitative Analysis Point-Centered Quarter Method
2010/11/15
This document is an introduction to the use of the point-centered quarter method. It briefly outlines its history, its methodology, and some of the practical issues and modifications that inevitably ...
应用实时定量RT-PCR技术检测b地中海贫血珠蛋白基因的表达Quantitative Analysis of Human Globin Gene Expression in b-thalassemia Using Real-Time RT-PCR
地中海贫血/遗传学 珠蛋白基因 基因表达 实时定量RT-PCR
2008/1/6
摘要
为了定量检测 b 地中海贫血(b 地贫)的 a、b 和γ珠蛋白基因表达水平， 提取正常成人对照组、正常胎儿对照组和b 地贫患者组组成的样本 DNA，采用反向点杂交法（RDB）分析b 地贫各种突变类型；提取样本RNA用于进行针对a、b 和γ珠蛋白基因的荧光实时定量RT-PCR（FQ RT-PCR）。根据FQ RT-PCR原理，设计合成分别对应于a、b 和γ珠蛋白基因的3对引物和3条荧光探针，...