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Efficacy of Bacillus thuringiensis Cry14 Toxin against Root Knot Nematode, Meloidogyne javanica
nematicidal nematode management tomato
2015/3/13
Two Bacillus thuringiensis strains including ToIr65 and ToIr67 with nematicidal activity against hatched juveniles and
eggs of Meloidogyne javanica were identified by phenotypic, microscopic, 16s rD...
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase, nuclease and gas vesicle genes in {\it Bacillus megaterium\/}
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase nuclease and gas vesicle genes in Bacillus megaterium
2015/1/28
To study polyhydroxyalkanoate (PHA) accumulation and PHA regulation in Bacillus megaterium, transposon mutagenesis with Tn917-LTV1 was carried out and seventy two mutants that produce more or less PHA...
Gene products from Bacillus megaterium involved in the metabolism of polyhydroxyalkanoic acid (PHA) and the biogenesis of PHA inclusion -bodies
Biological sciences Polyhydroxyalkanoic acid Inclusion-bodies Bacillus megaterium Biodegradable polymers
2015/1/28
Polyhydroxyalkanoates (PHAs) comprise a family of macromolecules produced by many bacteria as a carbon and energy reserve and are perceived to have commercial potential as biodegradable thermoplastics...
Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets
Bacterial Community Diarrhea Growth Performance Lactobacillus Weaned Piglets
2016/5/17
This research focused on the effects of different doses of Bacillus subtilis KN-42 on the growth performance, diarrhea incidence, faecal bacterial flora, and the relative number of Lactobacillus and E...
Production of Endoglucanase, Beta-glucosidase and Xylanase by Bacillus licheniformis Grown on Minimal Nutrient Medium Containing Agriculture Residues
Bacillus licheniformis β-Glucosidase Endoglucanase Xylanase
2016/5/16
Bacillus licheniformis was grown in minimal nutrient medium containing 1% (w/v) of distillers dried grain with soluble (DDGS), palm kernel meal (PKM), wheat bran (WB) or copra meal (CM), and the enzym...
Bacillus subtilis Protects Porcine Intestinal Barrier from Deoxynivalenol via Improved Zonula Occludens-1 Expression
Zonula Occludens-1 Porcine Intestinal Epithelial Cell Line (IPEC-J2)
2016/5/16
Intestinal epithelial cells (IECs) forming the barrier for the first-line of protection are interconnected by tight junction (TJ) proteins. TJ alteration results in impaired barrier function, which ca...
Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme
cyclodextrin glycosyltransferase affinity chromatography purification
2014/3/28
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enz...
The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers
Probiotic Bacillus subtilis Mucin Gene Expression Small Intestine Broilers
2016/5/10
The aim of the present study was to evaluate the effect of commercial monostrain and multistrain probiotics in diets on growth performance, intestinal morphology and mucin gene (MUC2) expression in br...
考察了不同搅拌转速、pH值和温度条件下,细胞生长、g-CGT酶产量与培养液中直链麦芽低聚糖积累的关系. 细胞的最大比生长速率为0.2 h-1是g-CGT酶产量从增长到下降的拐点. 发酵液中直链麦芽低聚糖的存在能促进细胞生长并引起胞内的FDP, ATP和G6P含量增加,而对产酶显示出分解代谢阻遏效应. 指数流加结果显示,通过限制细胞的比生长速率,可将阻遏作用控制在较低水平,酶产量得到了大幅度提高,是...
摘要从一个工业上生产。a-淀粉酶的枯草杆菌(Baciltus subtilis) BF--7658菌株中分离出自发的依赖链霉素、抗红霉素孢子形成突变体。这些突变体能正常营养生长,但不能正常形成孢子;同时蛋白酶、抗菌素活性降低,转化分析说明这些性状的改变是单一突变的结果。
摘要Staphylococcus aureus 307株是临床分离的一多重耐药味,其中包括抗氯霉素(Cm R),抗卡那霉素(KmR)和抗四环素(TcR)。实验结果表明,这三种抗药性是分别为三个质粒所决定的。我们把它们分别称为pC307、pK307和pT307。通过转化把三个质粒引入Bacillus subtilis Ki-2和168株,获得CmR KmR TcR转化体。本文研究了这些质粒所推带的抗...
摘要以诱变分离得到Bacillus subtilis W168核糖体蛋白质S3、S7、S8、S11、S13、BL2、BL6、BL7突变体。通过遗传分析定位了S13和BL18基因。S13基因(rps M)位于主要核糖体蛋白质基因群str A-spc A区中,而BL18基因(rpl S)和pyr D紧密连锁。BL18突变对细胞RNA和蛋白质合成以及噬菌体ф29的成斑率没有明显影响。
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细菌感受态细胞摄取和分泌DNA的相关性研究① I. 含有质粒的枯草芽孢杆菌自然感受态缺陷突变株的诱变和分离 Study of the Interrelations Between DNA Uptake and Secreting of Competent Bacterial Cells I. Mutagenesis and Isolation of Natural Competence-deficient Mutant from Containing Plasmid Bacillus subtilis
枯草芽孢杆菌 自然感受态缺陷突变株 诱变 筛选
2008/1/7
以携带pUB110质粒的枯草芽孢杆菌BR151菌株为出发菌株,利用亚硝基胍作诱变剂,直接在LB平板上进行诱变。从2949个菌落中筛选出一个转化频率低于出发菌株2~3个数量级的突变株,并对其营养缺陷型、UV的敏感性及Km抗性和质粒进行了检测,确证其转化能力降低为自然感受态缺陷而非营养缺陷型的改变或重组缺陷所致。