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Paper is at the heart of an experimental device developed by Rice University bioengineers to study heart disease.They are using paper-based structures that mimic the layered nature of aortic valves, t...
A meteoroid penetrating the Earth’s atmosphere leaves behind a trail of dense plasma embedded in the lower E/upper D-region ionosphere. While radar measurements of meteor trail evolution have been c...
High‐power large‐aperture (HPLA) radars frequently observe nonspecular meteor trail echoes that result from plasma turbulence driven by the intense pressure gradients on the trail edges. This paper ...
Reach back far enough in the family tree of a snail or a clam and you’ll find a spiny little slug with tiny teeth, wearing a helmet.Scientists have unearthed the 480-million-year-old remains of a crea...
NASA’s Cassini and Huygens missions have provided a wealth of data about chemical elements found on Saturn’s moon Titan, and Cornell scientists have uncovered a chemical trail that suggests prebiotic ...
Consider a graph with a set of vertices and oriented edges connecting pairs of vertices. Each vertex is associated with a random variable and these are assumed to be independent. In this setting, supp...
溶瘤腺病毒的肿瘤靶向性研究一直是一个热点。目前已有商业化的ONYX-015、H101溶瘤腺病毒。在此基础上, 科学家又进一步发展形成基因-病毒治疗方案, 如文中应用的ZD55-TRAIL病毒。本研究利用刘新垣实验室提供的携带TRAIL(TNF-relatedapoptosis-inducing ligand, TNF相关的凋亡诱导配体)的溶瘤腺病毒ZD55-TRAIL[1]联合雷帕霉素杀伤肿瘤细胞...
High‐power, large‐aperture (HPLA) radars have been used over the past two decades to characterize the plasmas formed both around and behind meteoroids as they enter Earth’s atmosphere. These plasmas...
The peculiar object P/2010 A2 was discovered by the LINEAR near-Earth asteroid survey in January 2010 and given a cometary designation due to the presence of a trail of material, although there was no...
摘要 肿瘤坏死因子相关的凋亡诱导配体 (TRAIL)能选择性诱导肿瘤细胞凋亡 .为利用基因工程技术获得重组TRAIL蛋白可溶性片段 (sTRAIL) ,设计 1对引物 .利用PCR技术特异性扩增出sTRAIL的cDNA ,克隆于质粒pGEM 3Zf( )的EcoRⅠ和PstⅠ位点 .经测序证明序列正确后克隆于表达质粒pBV2 2 0的EcoRⅠ和PstⅠ位点 ,转化大肠杆菌DH5α .转化菌株经温...
摘要以PCR方法克隆了TrailcDNA全长,构建了其真核表达载体,通过脂质体转染HeLa细胞,48小时后利用流式细胞仪分析Trail诱导细胞凋亡的比率,发现发生凋亡的细胞为总细胞数的19%。证实Trail真核表达系统的产物的生物学活性高,为从真核表达的途径获得Trail基因工程产品奠定了基础。
期刊信息 篇名 高密度发酵制备重组人可溶性TRAIL 语种 中文 撰写或编译 作者 王梁华,朱玉平,娄永华等 第一作者单位 刊物名称 第二军医大学学报 页面 2002;23(2): 132-135 出版日期 2002年 月 日 文章标识(ISSN) 相关项目 TRAIL对人肝癌细胞株杀伤效应敏感性差异的分子机理

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