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Journal of Chromatography A：提出多价灭活口蹄疫病毒疫苗抗原含量检测新策略
Journal of Chromatography A 多价疫苗 灭活口蹄疫病毒疫苗 抗原含量
2021/8/16
多价疫苗中每种血清型的有效抗原含量是影响疫苗质量的重要因素，以往检测技术存在无法分型和同时定量检测等问题。近日，中国科学院过程工程研究所生化工程国家重点实验室研究员张松平、苏志国团队利用毛细管区带电泳（Capillary Zone Electrophoresis，CZE）技术，实现了对O/A双价灭活口蹄疫病毒疫苗抗原（FMDV）中O型、A型完整病毒的同时定量检测，作为单价和双价FMDV疫苗质量控制...
中国科学院广西植物研究所李典鹏研究团队在国际学术期刊Journal of Chromatography A上发表论文（图）
中国科学院广西植物研究所 李典鹏 研究团队 国际 学术期刊 Journal of Chromatography A 论文
2019/6/13
日前，我所李典鹏研究团队基于广西壮族自治区八桂学者专项项目建设的高速逆流色谱平台，并在八桂学者Guido Frank Pauli教授及美国伊利诺伊大学芝加哥分校陈绍农博士的悉心指导下完成的研究论文“Preparation of flavone di-C-glycoside isomers from Jian-Gu Injection (Premna fulva Craib.) using recy...
Simultaneous determinations of fluorine, chlorine, and sulfur in rock samples by ion chromatography combined with pyrohydrolysis
pyrohydrolysis halogen sulfur ion chromatography geological reference material
2017/4/10
We present a simplified method for simultaneous determinations of fluorine (F), chlorine (Cl), and sulfur (S) contents
and isotopic abundances in rock samples. After using pyrohydrolysis to extract F...
Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
日前，我所刘昭前教授及其研究团队在《Biomedical Chromatography》上发表一篇能同时检测5种CYP450酶的探针底物的相应代谢产物的液相色谱-质谱（LC-MS/MS）的检测方法。该方法能同时检测5种CYP450酶的探针底物的相应代谢产物：7-羟基香豆素，CYP2A6；4-羟基甲苯磺丁脲，CYP2C9；4-羟基美芬妥因，CYP2C19；α-羟基美托洛尔，CYP2D6；和1-羟基咪...
Liquid chromatography/mass spectrometry analysis of perfluoroalkyl carboxylic acids and perfluorooctanesulfonate in bivalve shells: Extraction method optimization
Perfluorinated carboxylic acids Perfluorooctanesulfonate Shell Soft tissue Acid digestion Solid phase extraction
2013/12/12
Different extraction methods, including extraction by organic solvents with and without acetic acid digestion,and mixed inorganic acid digestion coupled with solid phase extraction (SPE), were develop...
Retention Behavior on Aminoethyl-modified Poly(p-phenylene terephthalamide) Fiber Stationary Phases in Gas Chromatography
Retention Behavior Aminoethyl-modified Poly(p-phenylene terephthalamide)
2010/9/17
Surface derivatization of Kevlar, poly(p-phenylene terephthalamide), fiber has been studied along with the evaluation of the surface characteristics of the chemically-modified fiber as the stationary ...
Trace Analysis of Polycyclic Aromatic Hydrocarbons Using Gas Chromatography–Mass Spectrometry Based on Nanosecond Multiphoton Ionization
Trace Analysis Polycyclic Aromatic Hydrocarbons Gas Chromatography–Mass Spectrometry Based Nanosecond Multiphoton Ionization
2010/9/15
Gas chromatography/resonance-enhanced multiphoton ionization/time-of-flight mass spectrometry (GC/REMPI-TOFMS) using an ultraviolet nanosecond laser was employed in the trace analysis of polycyclic ar...
Multifunctional Separation Mechanism on Poly(oxyethylene) Stationary Phases in Capillary Liquid Chromatography
Multifunctional Separation Mechanism Capillary Liquid Chromatography
2010/9/17
The retention behavior on poly(oxyethylene) (POE) chemically-bonded stationary phases was investigated in ion-exchange, reversed-phase (RP) and hydrophilic interaction (HILIC) modes in capillary liqui...
Mesoporous Platinum Electrodes for Amperometric Determination of Sugars with Anion Exchange Chromatography
Mesoporous Platinum Electrodes Amperometric Determination Sugars
2010/9/17
A simple amperometric detection (AD) method based on mesoporous platinum (Pt) electrodes has been developed for the determination of sugars (glucose, fructose, and sucrose) with high-performance anion...
Separation of Optical Isomers in Capillary Chromatography Using a Poly(tetrafluoroethylene) Capillary Tube and an Aqueous–Organic Mixture Carrier Solution
Separation Optical Isomers in Capillary Chromatography tetrafluoroethylene
2010/9/13
Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water–hydrophilic/hydrophobic organic solvent mixture as ...
Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography
Temperature-Programmed Separation Retention Prediction Novel Packed-Capillary Column
2010/9/13
Novel packed-capillary columns for gas chromatography were developed with a thin-wall stainless-steel capillary of 1.0 mm i.d.; and rapid temperature-programmed separations have been carried out after...
Rapid and Simultaneous Determination of Tetrafluoroborate, Thiocyanate and Hexafluorophosphate by High-Performance Liquid Chromatography Using a Monolithic Column and Direct Conductivity Detection
Rapid Simultaneous Determination Tetrafluoroborate
2010/9/15
A method was developed for fast and simultaneous determination of tetrafluoroborate (BF4−), thiocyanate (SCN−) and hexafluorophosphate (PF6−) by high-performance liquid chromatograph...
Analytical Conditions and Separation Performance of Capillary Chromatography Based on the Tube Radial Distribution of Aqueous–Organic Mixture Carrier Solvents under Laminar-Flow Conditions
Analytical Conditions Separation Performance Capillary Chromatography Based
2010/9/14
We have developed a capillary chromatography system using an open capillary tube made of fused-silica, polyethylene, or poly(tetrafluoroethylene), and a water–hydrophilic–hydrophobic organic mixture c...
Analyses of Non-steroidal Anti-inflammatory Drugs in Environmental Water Samples with Microemulsion Electrokinetic Chromatography
Non-steroidal Anti-inflammatory Drugs Environmental Water Samples Microemulsion Electrokinetic Chromatography
2010/9/13
In this study, a microemulsion electrokinetic chromatography (MEEKC) was used to analyze ten non-steroidal anti-inflammatory drugs (NSAIDs) in water samples. The type and ratio of organic modifiers we...